In most cases, the double-beam UV-Vis extinction spectra is taken as the light absorption of the material without considering the amount of light scattering. However, for large materials, like nanoparticles and supramolecules, their large size can cause significant light scattering contribution to the UV-Vis extinction spectra. In these cases, where light scattering is likely present, the UV-Vis Extinction spectra can't be deemed as light absorption because the amount of light scattering is unknown. In this work, a new Integrating-Sphere-Assisted Resonance Synchronous Spectroscopy (ISARS) spectroscopic method allows for the quantitative separation of light absorption and scattering contribution in typical double-beam UV-Vis extinction spectra by utilizing a conventional spectrofluorometer equipped with an integrating sphere accessory. This new spectroscopic method provides significant insight for the characterizing of emerging macromolecules, supramolecules, and nanoscale materials that are often simultaneous light absorbers, light scatterers, and/or light emitters. Big thanks to Dr. Juan Hu for their contribution to this work!
Comments